Rapid genotyping of three polymorphisms in the LBP gene using a triplex pyrosequencing approach

J Microbiol Methods. 2013 Sep;94(3):300-2. doi: 10.1016/j.mimet.2013.07.008. Epub 2013 Jul 20.

Abstract

We described a triplex polymerase chain reaction (PCR) and triplex pyrosequencing assay which allowed a simultaneous determination of three tag single nuleotide polymorphisms (tag SNPs) in the lipopolysaccharide-binding protein (LBP) gene: rs1780623, rs11536972 and rs2232618. This method enables a fast and cost-effective genotyping and a simultaneous determination of the three tag SNPs.

Keywords: Lipopolysaccharide-Binding Protein; Single Nuleotide Polymorphism; Triplex pyrosequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute-Phase Proteins / genetics*
  • Carrier Proteins / genetics*
  • Genotyping Techniques / methods*
  • Humans
  • Membrane Glycoproteins / genetics*
  • Polymorphism, Single Nucleotide / genetics*
  • Sequence Analysis, DNA / methods*

Substances

  • Acute-Phase Proteins
  • Carrier Proteins
  • Membrane Glycoproteins
  • lipopolysaccharide-binding protein