PAP/HIP protein is an obesogenic factor

J Cell Physiol. 2014 Feb;229(2):225-31. doi: 10.1002/jcp.24438.

Abstract

In this article we report the obesogenic role of the acute phase protein PAP/HIP. We found that the transgenic TgPAP/HIP mice develop spontaneous obesity under standard nutritional conditions, with high levels of glucose, leptin, and LDL and low levels of triglycerides and HDL in blood. Accordingly, PAP/HIP-deficient mice are skinny under standard nutritional conditions. We also found that expression of PAP/HIP is induced in intestinal epithelial cells in response to gavage with olive oil and this induction is AG490 sensitive. We demonstrated that incubation of 3T3-L1 preadipocytes with a low concentration as 1 ng/ml of recombinant PAP/HIP results in accelerated BrdU incorporation in vitro. PAP/HIP-dependent adipocytes growth is sensitive to the MEK inhibitor U0126. Finally, patients with severe obesity present higher blood levels of PAP/HIP than non-obese control individuals. Altogether our data suggest that PAP/HIP could be a mediator of fat tissue development, released by the intestine and induced by the presence of food into the gut.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipose Tissue / metabolism
  • Adult
  • Animals
  • Blood Coagulation Factors / genetics
  • Blood Coagulation Factors / metabolism*
  • Butadienes
  • Female
  • Gene Expression Regulation / physiology
  • Humans
  • Male
  • Mice
  • Mice, Knockout
  • Mice, Transgenic
  • Middle Aged
  • Nitriles
  • Obesity / genetics
  • Obesity / metabolism*
  • Pancreatitis-Associated Proteins
  • Proteins / genetics
  • Proteins / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins
  • Ribosomal Proteins

Substances

  • Blood Coagulation Factors
  • Butadienes
  • Nitriles
  • Pancreatitis-Associated Proteins
  • Proteins
  • RNA, Messenger
  • RNA-Binding Proteins
  • RPL29 protein, human
  • Reg3b protein, mouse
  • Ribosomal Proteins
  • U 0126