Laboratory investigation of an Escherichia coli O157:H7 strain possessing a vtx2c gene with an IS1203 variant insertion sequence isolated from an asymptomatic food handler in Japan

Tetsuya Harada; Yuji Hirai; Takeshi Itou; Mizuho Hayashida; Kazuko Seto; Masumi Taguchi; Yuko Kumeda

doi:10.1016/j.diagmicrobio.2013.06.012

Laboratory investigation of an Escherichia coli O157:H7 strain possessing a vtx2c gene with an IS1203 variant insertion sequence isolated from an asymptomatic food handler in Japan

Diagn Microbiol Infect Dis. 2013 Oct;77(2):176-8. doi: 10.1016/j.diagmicrobio.2013.06.012. Epub 2013 Jul 26.

Authors

Tetsuya Harada¹, Yuji Hirai, Takeshi Itou, Mizuho Hayashida, Kazuko Seto, Masumi Taguchi, Yuko Kumeda

Affiliation

¹ Division of Bacteriology, Osaka Prefectural Institute of Public Health, Osaka 537-0025, Japan. Electronic address: [email protected].

PMID: 23891550
DOI: 10.1016/j.diagmicrobio.2013.06.012

Abstract

We isolated an Escherichia coli O157:H7 strain that was negative for verocytotoxin production, but positive for the vtx2 gene using commercial kits, from an asymptomatic food handler. The laboratory investigations revealed that a 1310-bp insertion sequence, IS1203 variant, was present in the B subunit-coding region of the vtx2c gene.

Keywords: E. coli O157:H7; IS1203v.

Publication types

Case Reports

MeSH terms

Adult
Carrier State / microbiology*
DNA, Bacterial / analysis
DNA, Bacterial / genetics
Escherichia coli Infections / microbiology*
Escherichia coli O157 / genetics*
Escherichia coli O157 / pathogenicity
Female
Food Handling
Genes, Bacterial*
Humans
Mutagenesis, Insertional*
Shiga Toxins / genetics*
Young Adult

Substances

DNA, Bacterial
Shiga Toxins