Abstract
Many G proteins are involved in the plant defense responses against pathogen infection. With Arabidopsis as a model, this chapter describes the protocols commonly used for inoculating plants with Pseudomonas syringae (a bacterial pathogen) and Botrytis cinerea (a fungal pathogen) for analyzing disease resistance phenotypes caused by these pathogens. In addition, the procedures are provided for observation of the hypersensitive response triggered by avirulent strains of P. syringae and for analyzing pathogen-responsive gene expression and MAPK activation.
MeSH terms
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Arabidopsis / genetics*
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Botrytis / pathogenicity*
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Disease Resistance / genetics*
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GTP-Binding Proteins / genetics
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GTP-Binding Proteins / metabolism
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Gene Expression Regulation, Plant
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Mitogen-Activated Protein Kinase Kinases / genetics
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Plant Diseases / genetics
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Plant Diseases / microbiology
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Plant Leaves / genetics
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Plant Leaves / microbiology
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Plants, Genetically Modified / genetics
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Plants, Genetically Modified / metabolism
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Pseudomonas syringae / genetics
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Pseudomonas syringae / pathogenicity*
Substances
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Mitogen-Activated Protein Kinase Kinases
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GTP-Binding Proteins