Assessment of resistance to anoikis in ovarian cancer

Methods Mol Biol. 2013:1049:347-54. doi: 10.1007/978-1-62703-547-7_26.

Abstract

Anoikis, a form of programmed cell death that occurs due to cell detachment from the extracellular matrix, is a critical mechanism in preventing ectopic cell growth. Acquisition of resistance to anoikis is a prerequisite for epithelial ovarian cancer cells to survive in ascitic fluids before forming metastatic foci. Here we describe a colorimetric method for monitoring the resistance of anoikis of ovarian cancer cells in vitro.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anoikis / genetics*
  • Apoptosis / genetics
  • Ascitic Fluid / pathology
  • Cell Line, Tumor
  • Cell Proliferation
  • Colorimetry / methods*
  • Extracellular Matrix / metabolism
  • Extracellular Matrix / pathology
  • Female
  • Humans
  • Neoplasm Metastasis / pathology
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / pathology*