Abstract
We present a FISH-based method for detecting single-nucleotide variants (SNVs) in exons and introns on individual RNA transcripts with high efficiency. We used this method to quantify allelic expression in cell populations and in single cells, and also to distinguish maternal from paternal chromosomes in single cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles
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Cell Line, Tumor
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Exons
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Female
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Gene Expression Profiling / methods*
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Humans
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In Situ Hybridization, Fluorescence
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Introns
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Male
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Melanoma / genetics
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Optical Imaging / methods*
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Polymorphism, Single Nucleotide*
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Proto-Oncogene Proteins B-raf / genetics
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RNA / analysis
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Single-Cell Analysis / methods*
Substances
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RNA
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BRAF protein, human
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Proto-Oncogene Proteins B-raf