Inhibition of human leukocyte elastase. 2. Inhibition by substituted cephalosporin esters and amides

J Med Chem. 1990 Sep;33(9):2522-8. doi: 10.1021/jm00171a029.

Abstract

A variety of 7 alpha-methoxycephalosporin ester and amide sulfones were prepared and tested to determine the structure-activity relations for inhibition of human leukocyte elastase (HLE), a serine protease which has been implicated in several degenerative lung and tissue diseases. The most potent IC50 values were obtained with neutral, lipophilic derivatives, with the esters being more active than the amides. However, the best time-dependent inhibition in this series was observed with the p- and m-carboxybenzyl esters 7b and 7c. These results are discussed in terms of the proposed mechanism of inhibition as well as a molecular modeling study using the recently solved X-ray crystal structure of HLE.

MeSH terms

  • Amides / chemical synthesis*
  • Amides / pharmacology
  • Binding Sites / drug effects
  • Carboxylic Acids / chemical synthesis*
  • Cephalosporins / chemical synthesis*
  • Cephalosporins / pharmacology
  • Chemical Phenomena
  • Chemistry
  • Esters / chemical synthesis*
  • Esters / pharmacology
  • Humans
  • Leukocyte Elastase
  • Models, Molecular
  • Pancreatic Elastase / antagonists & inhibitors*
  • Structure-Activity Relationship

Substances

  • Amides
  • Carboxylic Acids
  • Cephalosporins
  • Esters
  • Pancreatic Elastase
  • Leukocyte Elastase