SEF14 fimbriae from Salmonella enteritidis play a role in pathogenitic to cell model in vitro and host in vivo

Chunhong Zhu; Xia Meng; Xiaoli Duan; Zhiyun Tao; Jiansen Gong; Huayan Hou; Guoqiang Zhu

doi:10.1016/j.micpath.2013.07.002

SEF14 fimbriae from Salmonella enteritidis play a role in pathogenitic to cell model in vitro and host in vivo

Microb Pathog. 2013 Nov:64:18-22. doi: 10.1016/j.micpath.2013.07.002. Epub 2013 Aug 7.

Authors

Chunhong Zhu¹, Xia Meng, Xiaoli Duan, Zhiyun Tao, Jiansen Gong, Huayan Hou, Guoqiang Zhu

Affiliation

¹ Jiangsu Institute of Poultry Science, Yangzhou 225125, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China.

PMID: 23933167
DOI: 10.1016/j.micpath.2013.07.002

Abstract

The role of SEF14 fimbriae in virulence remains to be elucidated and in this study, we showed that sefA mutant constructed in the wild-type (WT) Salmonella enteritidis strain 50336 displayed increased invasion to IPEC-J2 cell lines and survival in mouse peritoneal macrophages, and the lethal dose 50% (LD50) in 6-week-old Balb/c mice intra-peritoneally injected with WT S. enteritidis strain decreased significantly upon deletion of sefA indicating their role in virulence. Overall, these results demonstrated that expression of sefA of SEF14 fimbriae enhances S. enteritidis adhesion to epithelial cells and survival in macrophages and contributes to S. enteritidis virulence in mice.

Keywords: Pathogenesis; SEF14 fimbriae; Salmonella enteritidis; sefA gene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Epithelial Cells / microbiology
Fimbriae Proteins / metabolism*
Lethal Dose 50
Macrophages, Peritoneal / microbiology
Mice
Mice, Inbred BALB C
Salmonella Infections, Animal
Salmonella enteritidis / pathogenicity*
Swine
Virulence
Virulence Factors / metabolism*

Substances

Virulence Factors
sefA protein, Salmonella enteritidis
Fimbriae Proteins