Abstract
Hematopoiesis is regulated by transcription factors that induce cell fate and differentiation in hematopoietic stem cells into fully differentiated hematopoietic cell types. The transcription factor zinc finger protein 148 (Zfp148) interacts with the hematopoietic transcription factor Gata1 and has been implicated to play an important role in primitive and definitive hematopoiesis in zebra fish and mouse chimeras. We have recently created a gene-trap knockout mouse model deficient for Zfp148, opening up for analyses of hematopoiesis in a conventional loss-of-function model in vivo. Here, we show that Zfp148-deficient neonatal and adult mice have normal or slightly increased levels of hemoglobin, hematocrit, platelets and white blood cells, compared to wild type controls. Hematopoietic lineages in bone marrow, thymus and spleen from Zfp148 (gt/gt) mice were further investigated by flow cytometry. There were no differences in T-cells (CD4 and CD8 single positive cells, CD4 and CD8 double negative/positive cells) in either organ. However, the fraction of CD69- and B220-positive cells among lymphocytes in spleen was slightly lower at postnatal day 14 in Zfp148 (gt/gt) mice compared to wild type mice. Our results demonstrate that Zfp148-deficient mice generate normal mature hematopoietic populations thus challenging earlier studies indicating that Zfp148 plays a critical role during hematopoietic development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, CD / metabolism
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Antigens, Differentiation, T-Lymphocyte / metabolism
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B-Lymphocytes / cytology
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B-Lymphocytes / metabolism
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Bone Marrow / embryology
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Bone Marrow / growth & development
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Bone Marrow / metabolism*
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DNA-Binding Proteins / deficiency
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DNA-Binding Proteins / genetics*
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Flow Cytometry
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Gene Expression Regulation, Developmental
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Hematopoiesis / genetics*
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Lectins, C-Type / metabolism
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Leukocyte Common Antigens / metabolism
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Lymphocyte Count
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Mice
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Mice, 129 Strain
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Mice, Inbred C57BL
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Mice, Knockout
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Reverse Transcriptase Polymerase Chain Reaction
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Spleen / embryology
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Spleen / growth & development
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Spleen / metabolism*
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Thymus Gland / embryology
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Thymus Gland / growth & development
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Thymus Gland / metabolism*
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Time Factors
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Transcription Factors / deficiency
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Transcription Factors / genetics*
Substances
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Antigens, CD
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Antigens, Differentiation, T-Lymphocyte
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CD69 antigen
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DNA-Binding Proteins
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Lectins, C-Type
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Transcription Factors
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Zfp148 protein, mouse
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Leukocyte Common Antigens
Grants and funding
This work was supported by grants from Assar Gabrielsson's Foundation (V.I.S), the Swedish Research Council (P.L), Polysackaridforskning AB (P.L), the Swedish Cancer Foundation (P.L), the Swedish Heart-Lung Foundation (P.L), the University of Gothenburg (P.L), and by Lymphangiogenomics, an Integrated Project funded by the European Commission within its FP6 Program, under the thematic area "Life sciences, genomics and biotechnology for health" (contract no. LSHG-CT-2004-503573) (P.L). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.