Efficient detection of hydrogen bonds in dynamic regions of RNA by sensitivity-optimized NMR pulse sequences

Angew Chem Int Ed Engl. 2013 Sep 27;52(40):10487-90. doi: 10.1002/anie.201304391. Epub 2013 Aug 14.

Abstract

Improved Sensitivity: Efficient NMR experiments are presented for determining the secondary structure in large and dynamic RNAs using J-couplings across hydrogen bonds. The experiments provide up to eight-fold improved sensitivity and thus enable detection of base pairs in dynamic regions even in large RNAs.

Keywords: NMR spectroscopy; RNA dynamics; RNA secondary structure; base pairs; hydrogen bonds.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Hydrogen Bonding
  • Molecular Sequence Data
  • Molecular Weight
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Nucleic Acid Conformation
  • RNA / chemistry*

Substances

  • RNA