Abstract
The performance of a real-time PCR assay targeting the Tropheryma whipplei rpoB gene was evaluated using test strains and 1,236 clinical specimens in a national reference laboratory. The novel rpoB-PCR assay proved to be specific, revealed improved analytical sensitivity, and substantially accelerated detection of T. whipplei DNA in clinical specimens.
Publication types
-
Research Support, Non-U.S. Gov't
-
Validation Study
MeSH terms
-
Bacteriological Techniques / methods*
-
DNA-Directed RNA Polymerases / genetics*
-
Humans
-
Molecular Diagnostic Techniques / methods*
-
Real-Time Polymerase Chain Reaction / methods*
-
Sensitivity and Specificity
-
Time Factors
-
Tropheryma / genetics
-
Tropheryma / isolation & purification*
-
Whipple Disease / diagnosis*
-
Whipple Disease / microbiology
Substances
-
DNA-Directed RNA Polymerases
-
RNA polymerase beta subunit