Panton-Valentine leukocidin producing Staphylococcus aureus nasal carriage, in North-Lebanon

Med Mal Infect. 2013 Sep;43(9):386-90. doi: 10.1016/j.medmal.2013.07.005. Epub 2013 Aug 20.

Abstract

Objectives: We investigated the prevalence and the risk factors of nasal carriage of Staphylococcus aureus carrying Panton-Valentine leukocidin genes [Sa PVL(+)] in pupils.

Study population and methods: Two hundred and fifty-seven pupils were screened by nasal swabbing. The detection of 16S rRNA, mecA and luk-PV genes was performed by PCR and the risk factors were assessed with the statistical analysis of a questionnaire.

Results: Thirty-one percent of pupils were colonized, with 16.4% of isolates carrying the luk-PV gene and 8.8% the mecA gene. Children aged 7years or more and living in a boarding school were the factors promoting nasal carriage 60% of children who presented with an abscess in the previous year were carriers of luk-PV gene Sa.

Conclusion: The study revealed a high prevalence of luk-PV gene among methicillin-susceptible strains and a statistically significant correlation between the presence of this gene and presenting with an abscess.

Keywords: Leucocidine de Panton-Valentine; Nasal carriage; Panton-Valentine leukocidin; Portage nasal; S. aureus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Bacterial Proteins / genetics
  • Bacterial Toxins / analysis*
  • Bacterial Toxins / genetics
  • Carrier State / epidemiology*
  • Carrier State / microbiology
  • Child
  • Child, Preschool
  • Exotoxins / analysis*
  • Exotoxins / genetics
  • Female
  • Furunculosis / microbiology
  • Genes, Bacterial
  • Housing
  • Humans
  • Lebanon / epidemiology
  • Leukocidins / analysis*
  • Leukocidins / genetics
  • Male
  • Methicillin / pharmacology
  • Nasal Cavity / microbiology*
  • Penicillin-Binding Proteins
  • Prevalence
  • Ribotyping
  • Schools
  • Staphylococcal Infections / epidemiology*
  • Staphylococcal Infections / microbiology
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics
  • Staphylococcus aureus / isolation & purification*
  • Virulence

Substances

  • Bacterial Proteins
  • Bacterial Toxins
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin
  • Penicillin-Binding Proteins
  • mecA protein, Staphylococcus aureus
  • Methicillin