Abstract
Recent advances have led to several systems to study transcription from defined loci in living cells. It has now become possible to address long-standing questions regarding the interplay between the processes of DNA damage repair and transcription-two disparate processes that can occur on the same stretch of chromatin and which both lead to extensive chromatin change. Here we describe the development of a system to create enzymatically induced DNA double-strand breaks (DSBs) at a site of inducible transcription and methods to study the interplay between these processes.
MeSH terms
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Animals
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Anti-Bacterial Agents / pharmacology
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Antineoplastic Agents / pharmacology
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Cell Line
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Chromatin / genetics
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Chromatin Assembly and Disassembly / physiology
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DNA Breaks, Double-Stranded*
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DNA Repair / genetics*
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DNA Repair / physiology
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Doxycycline / pharmacology
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Humans
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Lac Operon / genetics
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Lasers
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Microscopy, Fluorescence / methods*
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Tamoxifen / analogs & derivatives
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Tamoxifen / pharmacology
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Transcription, Genetic / genetics*
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Transcription, Genetic / physiology
Substances
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Anti-Bacterial Agents
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Antineoplastic Agents
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Chromatin
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Tamoxifen
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afimoxifene
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endodeoxyribonuclease FokI
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Deoxyribonucleases, Type II Site-Specific
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Doxycycline