Abstract
In this study, proteins specifically interacting with the 3' untranslated region (UTR) of mRNA of the multifunctional Y-box-binding protein 1 (YB-1) were identified. One of these, hnRNP Q, was shown to specifically interact with the regulatory element (RE) in YB-1 mRNA 3' UTR and to inhibit translation of this mRNA. Its binding to the RE was accompanied by displacement from this element of the poly(A)-binding protein (PABP), a positive regulator of YB-1 mRNA translation, and by enhanced binding of the negative YB-1 mRNA translation regulator - YB-1 itself.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Animals
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Cell Line, Tumor
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Cell-Free System
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HEK293 Cells
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HeLa Cells
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Heterogeneous-Nuclear Ribonucleoproteins / chemistry
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Heterogeneous-Nuclear Ribonucleoproteins / genetics
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Heterogeneous-Nuclear Ribonucleoproteins / metabolism*
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Humans
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Protein Binding
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Protein Biosynthesis
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RNA, Messenger / chemistry
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RNA, Messenger / metabolism*
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Rabbits
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Regulatory Elements, Transcriptional
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Y-Box-Binding Protein 1 / genetics
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Y-Box-Binding Protein 1 / metabolism*
Substances
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3' Untranslated Regions
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Heterogeneous-Nuclear Ribonucleoproteins
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RNA, Messenger
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Recombinant Proteins
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Y-Box-Binding Protein 1