Abstract
Monomeric hIAPP significantly destabilizes both model and live cell membranes by increasing membrane fluidity. This interaction with membranes happens via carpet formation followed by lipid extraction in a concentration dependent manner and thus we propose that hIAPP aggregation prior to membrane interaction may not be necessary for its cytotoxicity.
MeSH terms
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Apoptosis / drug effects
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Cell Line, Tumor
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Cell Membrane / chemistry
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Cell Membrane / metabolism*
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Circular Dichroism
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Humans
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Islet Amyloid Polypeptide / chemistry
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Islet Amyloid Polypeptide / metabolism*
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Islet Amyloid Polypeptide / toxicity
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Lipid Bilayers / chemistry
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Lipid Bilayers / metabolism*
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Membrane Fluidity
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Microscopy, Fluorescence
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Monte Carlo Method
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Protein Structure, Secondary
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Rhodamines / chemistry
Substances
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Islet Amyloid Polypeptide
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Lipid Bilayers
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Rhodamines