Phenylpyrazolo[1,5-a]quinazolin-5(4H)-one: a suitable scaffold for the development of noncamptothecin topoisomerase I (Top1) inhibitors

J Med Chem. 2013 Sep 26;56(18):7458-62. doi: 10.1021/jm400932c. Epub 2013 Sep 16.

Abstract

In search for a novel chemotype to develop topoisomerase I (Top1) inhibitors, the pyrazolo[1,5-a]quinazoline nucleus, structurally related to the indenoisoquinoline system precursor of well-known Top1 poisons, was variously decorated (i.e., a substituted phenyl ring at 2- or 3-position, a protonable side chain at 4- or 5-position), affording a number of Top1 inhibitors with cleavage patterns common to CPT and MJ-III-65. SARs data were rationalized by means of an advanced docking protocol.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Topoisomerases, Type I / chemistry
  • DNA Topoisomerases, Type I / metabolism*
  • Drug Discovery*
  • Heterocyclic Compounds, 3-Ring / chemistry
  • Heterocyclic Compounds, 3-Ring / metabolism
  • Heterocyclic Compounds, 3-Ring / pharmacology*
  • Humans
  • Molecular Docking Simulation
  • Protein Conformation
  • Pyrroles / chemistry*
  • Pyrroles / metabolism
  • Pyrroles / pharmacology*
  • Quinazolines / chemistry*
  • Quinazolines / metabolism
  • Quinazolines / pharmacology*
  • Topoisomerase I Inhibitors / chemistry*
  • Topoisomerase I Inhibitors / metabolism
  • Topoisomerase I Inhibitors / pharmacology*

Substances

  • Heterocyclic Compounds, 3-Ring
  • Pyrroles
  • Quinazolines
  • Topoisomerase I Inhibitors
  • DNA Topoisomerases, Type I