Abstract
The Hippo (Hpo) pathway controls tissue growth and organ size by regulating the activity of transcriptional co-activator Yorkie (Yki), which associates with transcription factor Scalloped (Sd) in the nucleus to promote downstream target gene expression. Here we identify a novel protein Sd-Binding-Protein (SdBP)/Tgi, which directly competes with Yki for binding to Sd through its TDU domains and inhibits the Sd-Yki transcriptional activity. We also find that SdBP retains Yki in the nucleus through the association with Yki WW domains via its PPXY motifs. Collectively, we identify SdBP as a novel component of the Hpo pathway, negatively regulating the transcriptional activity of Sd-Yki to restrict tissue growth.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Animals
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Carrier Proteins / analysis
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Line
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Drosophila / genetics
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Drosophila / growth & development*
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Drosophila / metabolism
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Drosophila Proteins / analysis
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism*
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Gene Expression Regulation, Developmental
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Intracellular Signaling Peptides and Proteins / metabolism*
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Nuclear Proteins / analysis
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Nuclear Proteins / metabolism*
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Protein Interaction Maps
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Protein Serine-Threonine Kinases / metabolism*
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Protein Structure, Tertiary
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Signal Transduction
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Trans-Activators / analysis
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Trans-Activators / metabolism*
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Transcription Factors / analysis
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Transcription Factors / metabolism*
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Transcriptional Activation
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Up-Regulation
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YAP-Signaling Proteins
Substances
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Carrier Proteins
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Drosophila Proteins
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Intracellular Signaling Peptides and Proteins
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Nuclear Proteins
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Tgi protein, Drosophila
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Trans-Activators
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Transcription Factors
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YAP-Signaling Proteins
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Yki protein, Drosophila
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sd protein, Drosophila
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Protein Serine-Threonine Kinases
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hpo protein, Drosophila