The aim of the investigation was to determine diversity of the causative agents of dermatophytoses and characterize the epidemiological situation in Lithuania in 2001-2010. During this period, dermatophytes showed a tendency to decline. The following dermatophytes were isolated: Trichophyton (T.) Malamsten, Microsporum (M.) Gruby and Epidermophyton E. Lang. The number of nondermatophytes increased. At the beginning of the investigation, nondermatophytes accounted for 3.4%, whereas at the end their number grew up to 35.9%. Among the agents of dermatomycosis, the incidence of yeasts was observed to have a growing tendency. Among dermatophytes, T. rubrum was the most common pathogen, which in 2001 amounted to 55.7% and in 2010 to only 11.0%. Among the Microsporum species, M. canis and M. gypseum were detected. A small number of Epidermophyton species were observed in 2001-2003, which accounted for 0.2%-0.8% of all isolates. Tinea unguium (75.5%) was the most common type of dermatophytosis, followed by tinea capitis (11.7%), tinea corporis (9.2%) and tinea pedis (1.2%). In 2001-2010, dermatophytes showed a decreasing tendency, whereas the incidence of Candida yeasts and other causative agents of dermatomycosis greatly increased.