Isolation of genomic DNA from mammalian cells

Cheryl M Koh

doi:10.1016/B978-0-12-418687-3.00013-6

Isolation of genomic DNA from mammalian cells

Methods Enzymol. 2013:529:161-9. doi: 10.1016/B978-0-12-418687-3.00013-6.

Author

Cheryl M Koh¹

Affiliation

¹ Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: [email protected].

PMID: 24011044
DOI: 10.1016/B978-0-12-418687-3.00013-6

Abstract

The isolation of genomic DNA from mammalian cells is a routine molecular biology laboratory technique with numerous downstream applications. The isolated DNA can be used as a template for PCR, cloning, and genotyping and to generate genomic DNA libraries. It can also be used for sequencing to detect mutations and other alterations, and for DNA methylation analyses.

Keywords: Cell lysis; Collection of mammalian cells; Ethanol precipitation; Isolation of genomic DNA; Mammalian cells; Organic extraction.

MeSH terms

Animals
DNA / isolation & purification*
Genome
Mammals / genetics*

Substances

DNA