Supraglottic laryngeal tumor microenvironmental factors facilitate STAT3 dependent pro-tumorigenic switch in tumor associated macrophages to render utmost immune evasion

Kuntal Kanti Goswami; Subhasis Barik; Saptak Banerjee; Anup Kumar Bhowmick; Jaydip Biswas; Anamika Bose; Rathindranath Baral

doi:10.1016/j.imlet.2013.09.003

Supraglottic laryngeal tumor microenvironmental factors facilitate STAT3 dependent pro-tumorigenic switch in tumor associated macrophages to render utmost immune evasion

Immunol Lett. 2013 Nov-Dec;156(1-2):7-17. doi: 10.1016/j.imlet.2013.09.003. Epub 2013 Sep 9.

Authors

Kuntal Kanti Goswami¹, Subhasis Barik, Saptak Banerjee, Anup Kumar Bhowmick, Jaydip Biswas, Anamika Bose, Rathindranath Baral

Affiliation

¹ Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute (CNCI), 37, S. P. Mukherjee Road, Kolkata 700026, India.

PMID: 24029664
DOI: 10.1016/j.imlet.2013.09.003

Abstract

Content of tumor microenvironment (TME) is varied greatly among different types of laryngeal tumors, namely, supraglottic, glottic and subglottic tumors. These three different TMEs shape infiltrating monocytes/macrophages toward M2 genotypes in variable degrees. Results obtained from in vitro studies demonstrated extent of expression of M2 phenotypic features on macrophages was maximum after their exposure to supraglottic laryngeal tumor cell lysates (SLTCL) than glottic or subglottic lysates. Moreover, M2 macrophages generated under influence of SLTCL show less nitric oxide production, greater IL-10: IL-12 ratio and poor antigen presentation. Co-culture of such M2 macrophages with T cells from healthy donors resulted decreased activation of T cells and T cell mediated tumor cell cytotoxicity, than, glottic or subglottic. SLTCL mediated macrophage polarization is STAT3 dependent and might be one of the major factors for severe immune paralysis leading to poor prognosis of supraglottic laryngeal tumor bearer following standard treatment.

Keywords: IL-10; M2 macrophages; Prognosis; STAT3; Supraglottic laryngeal tumor.

MeSH terms

Animals
Antigen Presentation / drug effects
Antigen Presentation / immunology
Cell Line, Tumor
Cells, Cultured
Coculture Techniques
Cytotoxicity, Immunologic
Epiglottis / metabolism
Epiglottis / pathology
Glottis / metabolism
Glottis / pathology
Humans
Immune Evasion / drug effects
Immune Evasion / immunology
Immunohistochemistry
Immunologic Factors / metabolism*
Immunologic Factors / pharmacology
Interleukin-10 / genetics
Interleukin-10 / immunology
Interleukin-10 / metabolism
Interleukin-12 / genetics
Interleukin-12 / immunology
Interleukin-12 / metabolism
Laryngeal Neoplasms / metabolism*
Laryngeal Neoplasms / pathology
Lymphocyte Activation / immunology
Macrophages / drug effects
Macrophages / immunology*
Macrophages / metabolism
Male
Nitric Oxide / immunology
Nitric Oxide / metabolism
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
STAT3 Transcription Factor / genetics
STAT3 Transcription Factor / immunology*
STAT3 Transcription Factor / metabolism
T-Lymphocytes / immunology
Tissue Extracts / metabolism*
Tissue Extracts / pharmacology
Tumor Microenvironment

Substances

Immunologic Factors
STAT3 Transcription Factor
Tissue Extracts
Interleukin-10
Interleukin-12
Nitric Oxide