Simultaneous online enrichment and identification of trace species based on microfluidic droplets

Anal Chem. 2013 Oct 15;85(20):9617-22. doi: 10.1021/ac4018082. Epub 2013 Sep 27.

Abstract

A facile online enrichment protocol has been proposed based on microfluidic droplets acting as an interface between a liquid chromatography separation system and detection systems of ESI-MS/MS and laser-induced fluorescence. Low-abundance species were successfully concentrated and analyzed in this system via droplet shrinkage. The proposed platform significantly increased the enrichment efficiency and detection sensitivity with reduced sample handling steps, short analysis time, and no cross-contamination. The presented system is universal, shows no discrimination, and is easily coupled with other separation and detection approaches.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Analytic Sample Preparation Methods / methods*
  • Animals
  • Chromatography, High Pressure Liquid
  • Limit of Detection
  • Microfluidic Analytical Techniques / methods*
  • Online Systems
  • Peptide Fragments / analysis
  • Peptide Fragments / chemistry
  • Peptide Fragments / isolation & purification
  • Serum Albumin, Bovine / chemistry
  • Spectrometry, Mass, Electrospray Ionization
  • Time Factors

Substances

  • Peptide Fragments
  • Serum Albumin, Bovine