Northern blotting

Methods Enzymol. 2013:530:75-87. doi: 10.1016/B978-0-12-420037-1.00003-8.

Abstract

To measure the size and amount of RNA transcribed from a specific gene of interest.

Keywords: Denaturing agarose gel electrophoresis; Hybridization; Nylon membrane; Radiolabeled RNA probe; Transfer RNA.

MeSH terms

  • Animals
  • Blotting, Northern / instrumentation
  • Blotting, Northern / methods*
  • Electrophoresis, Agar Gel / instrumentation
  • Electrophoresis, Agar Gel / methods
  • Nucleic Acid Hybridization / methods
  • RNA / analysis*
  • RNA / genetics

Substances

  • RNA