Outbreaks of highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) have led to large economic losses and, subsequently, have drawn great attention to its diagnosis and prevention. To facilitate rapid discrimination of HP-PRRSV from classical PRRSV (C-PRRSV), we developed a one-step RT-PCR assay. Primer specificities were evaluated with RNA extracted from 8 viral strains and our results revealed that the primers had a high specificity for PRRSV. The assay sensitivity was 25 copies/μL for both HP-PRRSV and C-PRRSV. A total of 929 serum samples were identified, of which 20.45% were HP-PRRSV-positive and 1.51% were C-PRRSV-positive, which was completely consistent with that of immunochromatochemistry and sequencing method. The proposed assay can detect the virus 2 days prior the onset of symptoms and it can be performed in 2h, thereby providing a rapid method to discriminate HP-PRRSV from C-PRRSV for the identification and prevention of PRRSV infections.
Keywords: CSFV; Highly pathogenic; JEV; Japanese encephalitis virus; One-step RT-PCR; PCV2; PPV; PRRSV; PRV; Porcine reproductive and respiratory syndrome virus; RV; Viral discrimination; classical swine fever virus; porcine circovirus type 2; porcine parvovirus; porcine reproductive and respiratory syndrome virus; pseudorabies virus; rotavirus.
© 2013.