Background & aims: Infection with hepatitis C virus is a worldwide health problem. An inadequate Th2 cytokine response promotes the fibrosis-cirrhosis fate. Immune-modulating molecules favoring a Th2 profile, such as HLA-G molecules of the HLA class Ib family, may play a role in chronic hepatitis. HLA-G contributes to the escape of tumors, and their involvement in viral infections has been increasingly described. The aim of this work was to study the expression of HLA-G in the liver, its cellular source and its regulation in cases of chronic C hepatitis.
Methods: HLA-G cells in blocks of liver derived from patients infected with HCV were labeled by immunohistochemistry and enumerated. Double immunofluorescence allowed the identification of the cellular source. HLA-G secretion by a human mast cell line was quantified by ELISA after various stimulations. After treatment with IFN-α, real-time PCR was performed to determine the kinetics of cytokine expression profiles, followed by heat map clustering analysis.
Results: The number of HLA-G+ cells was significantly associated with the area of fibrosis. For the first time, we identify the HLA-G+ cells as being mast cells. HLA-G secretion was significantly induced in human mast cells stimulated by IL-10 or interferons of class I. The transcriptome of the secretome of this cell line stimulated by IFN-α revealed that (i) the HLA-G gene is upregulated late, and that (ii) T lymphocytes and NK cells are recruited.
Conclusions: These findings suggest an autocrine loop in the genesis of HCV liver fibrosis, based on mast cells expressing HLA-G.
Keywords: Fibrosis; HBV; HCC; HCMV; HCV; HIV; HLA-G; HSC; Hepatitis C; IFN; IFN-α; IL; ILT; Immunoglobulin-like transcript receptors; Liver; MC; Mast cell; OSM; SCF; TGF-β; hepatic stellate cell; hepatitis B virus; hepatitis C virus; hepatocellular carcinoma; human cytomegalovirus; human immunodeficiency virus; interferon; interleukin; mast cell; oncostatin; stem cell factor; transforming growth factor β.
Copyright © 2013 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.