2,4-Diamino-6,7-dimethylpteridine as a fluorescent ligand for binding and sensing an orphan cytosine in RNA duplexes

Chem Commun (Camb). 2013 Nov 4;49(85):9983-5. doi: 10.1039/c3cc46085a.

Abstract

We report on a new fluorescent ligand, 2,4-diamino-6,7-dimethylpteridine, that can strongly and selectively bind to an orphan cytosine opposite an abasic site in RNA duplexes. We describe a significant effect of the substituents attached to the pteridine ring on the binding behavior in comparison with a structurally-similar pteridine derivative.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Cytosine / chemistry*
  • Cytosine / metabolism
  • Ligands
  • Molecular Structure
  • Pteridines / chemistry*
  • Pteridines / metabolism
  • RNA / chemistry*
  • Spectrometry, Fluorescence
  • Thermodynamics

Substances

  • Ligands
  • Pteridines
  • 2,4-diamino-6,7-dimethylpteridine
  • RNA
  • Cytosine