Antibacterial hydrogel coating by electrophoretic co-deposition of chitosan/alkynyl chitosan

Carbohydr Polym. 2013 Nov 6;98(2):1547-52. doi: 10.1016/j.carbpol.2013.07.042. Epub 2013 Jul 29.

Abstract

Despite much effort has been paid to develop aseptic implant devices, the infection associated with medical implant still remains a significant problem. Here, we report a potential coating material derived from a natural biopolymer chitosan. Firstly, chitosan functionalized with alkynyl moiety (ACS) was prepared by reaction between chitosan and 3-bromopropyne. The structure of the alkynyl chitosan was characterized by FT-IR, (1)H NMR, XRD, TGA and element analysis. The minimum inhibitory concentration (MIC) of ACS with a degree of substitution (DS) of 0.40 was 0.03% against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Subsequently, the alkynyl chitosan was co-deposited with chitosan on stainless steel wire to fabricate a composite hydrogel. The composite hydrogel exhibited better antibacterial activities than pure chitosan hydrogel.

Keywords: Alkynyl chitosan; Antibacterial activity; Chitosan; Composite hydrogel; Electrophoretic co-deposition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / chemistry*
  • Anti-Bacterial Agents / chemistry*
  • Anti-Bacterial Agents / pharmacology
  • Chitosan / analogs & derivatives*
  • Chitosan / chemistry
  • Chitosan / pharmacology
  • Coated Materials, Biocompatible / chemistry*
  • Coated Materials, Biocompatible / pharmacology
  • Electrophoresis
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Humans
  • Hydrogels
  • Microbial Sensitivity Tests
  • Microscopy, Electron, Scanning
  • Stainless Steel
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / growth & development

Substances

  • Alkynes
  • Anti-Bacterial Agents
  • Coated Materials, Biocompatible
  • Hydrogels
  • Stainless Steel
  • Chitosan