Abstract
MicroRNAs (miRNAs) have been demonstrated to play a pivotal role in the regulation of target gene expression at the post-transcriptional level. In order to better understand the role of miRNA in the immunological regulation of macrophages against Mycobacterium bovis BCG infection, we explored the alteration of immune-related miRNA profile in macrophage RAW264.7 cells in response to BCG infection in this study. Our results demonstrated that miR-142-3p was a potential to negatively regulate the production of pro-inflammatory mediators NF-κB (NF-κB1), TNF-α and IL-6 in the macrophages in part through a mechanism of targeting IRAK-1 gene and post-transcriptionally down-regulating IRAK-1 protein expression.
Keywords:
BCG; IRAK-1; Macrophage; Mycobacterium; microRNA.
Copyright © 2013 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cells, Cultured
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Down-Regulation / genetics
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Down-Regulation / immunology
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Immunity, Innate / genetics
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Immunity, Innate / immunology
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Inflammation Mediators / metabolism
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Interleukin-1 Receptor-Associated Kinases / biosynthesis*
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Interleukin-1 Receptor-Associated Kinases / genetics
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Interleukin-6 / biosynthesis
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Interleukin-6 / genetics
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Macrophages / immunology
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Macrophages / microbiology*
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Mice
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MicroRNAs / genetics*
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Mycobacterium bovis*
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NF-kappa B / biosynthesis
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NF-kappa B / genetics
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RNA, Messenger / genetics
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Transfection
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Tuberculosis / genetics*
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Tuberculosis / immunology
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Tuberculosis / metabolism
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Tumor Necrosis Factor-alpha / biosynthesis
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Tumor Necrosis Factor-alpha / genetics
Substances
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Inflammation Mediators
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Interleukin-6
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MicroRNAs
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Mirn142 microRNA, mouse
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NF-kappa B
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Interleukin-1 Receptor-Associated Kinases
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Irak1 protein, mouse