Abstract
Arabidopsis CTD-PHOSPHATASE-LIKE 1 (CPL1) is a protein phosphatase that can dephosphorylate RNA polymerase II C-terminal domain (CTD). Unlike typical CTD-phosphatases, CPL1 contains a double-stranded (ds) RNA-binding motif (dsRBM) and has been implicated for gene regulation mediated by dsRNA-dependent pathways. We investigated the role of CPL1 and its dsRBMs in various gene silencing pathways. Genetic interaction analyses revealed that cpl1 was able to partially suppress transcriptional gene silencing and DNA hypermethylation phenotype of ros1 suggesting CPL1 is involved in the RNA-directed DNA methylation pathway without reducing siRNA production. By contrast, cpl1 reduced some miRNA levels at the level of processing. Indeed, CPL1 protein interacted with proteins important for miRNA biogenesis, suggesting that CPL1 regulates miRNA processing. These results suggest that CPL1 regulates DNA methylation via a miRNA-dependent pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / enzymology*
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Arabidopsis / genetics*
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Arabidopsis / physiology
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Cell Nucleus / metabolism
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Cold Temperature
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DNA Methylation / genetics*
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Gene Expression Regulation, Plant
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Gene Silencing
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Genetic Loci / genetics
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Mutation / genetics
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Phenotype
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Phosphoprotein Phosphatases / genetics
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Phosphoprotein Phosphatases / metabolism*
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Protein Binding
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RNA, Plant / genetics
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RNA, Plant / metabolism
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RNA-Binding Proteins / genetics
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RNA-Binding Proteins / metabolism*
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Stress, Physiological / genetics
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Suppression, Genetic
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription, Genetic
Substances
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Arabidopsis Proteins
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MicroRNAs
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RNA, Plant
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RNA-Binding Proteins
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Transcription Factors
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CPL1 protein, Arabidopsis
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Phosphoprotein Phosphatases
Grants and funding
This work was supported by grants from the BK21 program of the Ministry of Education and Science Technology of Korea, the National Science Foundation (MCB0950459), USDA-CSREES (2009-34402-19831) “Designing Food for Health”, and Grant-in-Aid for Scientific Research (number 24570044) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.