Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients

Patricia Costaglioli; Christophe Barthe; Michael Fayon; Naik Christoflour; Stéphanie Bui; Laurence Derlich; Philippe Domblides; Marc Crouzet; Sébastien Vilain; Bertrand Garbay

doi:10.1016/j.mcp.2013.09.003

Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients

Mol Cell Probes. 2014 Feb;28(1):10-2. doi: 10.1016/j.mcp.2013.09.003. Epub 2013 Sep 25.

Authors

Patricia Costaglioli¹, Christophe Barthe, Michael Fayon, Naik Christoflour, Stéphanie Bui, Laurence Derlich, Philippe Domblides, Marc Crouzet, Sébastien Vilain, Bertrand Garbay

Affiliation

¹ Univ. Bordeaux, Biotechnologie des Protéines Recombinantes à Visée Santé, EA 4135, F-33000 Bordeaux, France. Electronic address: [email protected].

PMID: 24075879
DOI: 10.1016/j.mcp.2013.09.003

Abstract

The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.

Keywords: Cystic fibrosis; Pseudomonas aeruginosa; RT-qPCR; Reference genes; Sputum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cystic Fibrosis / microbiology*
Gene Expression Profiling / methods
Genes, Bacterial*
Humans
Pseudomonas aeruginosa / genetics*
Real-Time Polymerase Chain Reaction
Sputum / microbiology*