Plant-derived mAbs have effective anti-cancer activities by increasing ganglioside expression in colon cancers

Biotechnol Lett. 2013 Dec;35(12):2031-8. doi: 10.1007/s10529-013-1318-z.

Abstract

An epithelial cell adhesion molecule (EpCAM) was selectively expressed in human colorectal carcinoma. Treatment with plant-derived anti-EpCAM mAb (mAbP CO17-1A) and RAW264.7 cells inhibited cell growth in the human colorectal cancer cell line SW620. In SW620 treated with mAbP CO17-1A and RAW264.7 cells, expression of p53 and p21 increased, whereas the expression of G1 phase-related proteins, cyclin D1, CDK4, cyclin E, and CDK2, decreased, similar to mammalian-derived mAb (mAbM) CO17-1A. Similar to mAbM CO17-1A, treatment with mAbP CO17-1A and RAW264.7 cell decreased the expression of anti-apoptotic protein, Bcl-2, but the expression of pro-apoptotic proteins Bax, TNF-α, caspase-3, caspase-6, caspase-8 and caspase-9, increased. Cells treated with mAbP CO17-1A and RAW264.7 cells expressed metastasis-related gangliosides, GM1 and GD1a, similar to mAbM CO17-1A. These results suggest that mAbP CO17-1A is as effective on anti-cancer activity as mAbM CO17-1A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / pharmacology*
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Colonic Neoplasms / metabolism*
  • Gangliosides / metabolism*
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • Plant Proteins / pharmacology*
  • Plants, Genetically Modified / metabolism
  • Recombinant Proteins / pharmacology

Substances

  • Antibodies, Monoclonal
  • Antineoplastic Agents
  • Gangliosides
  • Plant Proteins
  • Recombinant Proteins