Nature and mediators of parietal epithelial cell activation in glomerulonephritides of human and rat

Am J Pathol. 2013 Dec;183(6):1769-1778. doi: 10.1016/j.ajpath.2013.08.008. Epub 2013 Oct 1.

Abstract

Bowman's capsule parietal epithelial cell activation occurs in several human proliferative glomerulonephritides. The cellular composition of the resulting hyperplastic lesions is controversial, although a population of CD133(+)CD24(+) progenitor cells has been proposed to be a major constituent. Mediator(s) involved in proliferation and migration of progenitor cells into the Bowman's space have been poorly explored. In a series of 36 renal biopsies of patients with proliferative and nonproliferative glomerulopathies, dysregulated CD133(+)CD24(+) progenitor cells of the Bowman's capsule invade the glomerular tuft exclusively in proliferative disorders. Up-regulation of the CXCR4 chemokine receptor on progenitor cells was accompanied by high expression of its ligand, SDF-1, in podocytes. Parietal epithelial cell proliferation might be sustained by increased expression of the angiotensin II (Ang II) type-1 (AT1) receptor. Similar changes of CXCR4, SDF-1, and AT1 receptor expression were found in Munich Wistar Frömter rats with proliferative glomerulonephritis. Moreover, an angiotensin-converting enzyme inhibitor normalized CXCR4 and AT1 receptor expression on progenitors concomitant with regression of crescentic lesions in a patient with crescentic glomerulonephritis. These results suggest that glomerular hyperplastic lesions derive from the proliferation and migration of renal progenitors in response to injured podocytes. The Ang II/AT1 receptor pathway may participate, together with SDF-1/CXCR4 axis, to the dysregulated response of renal precursors. Thus, targeting the Ang II/AT1 receptor/CXCR4 pathways may be beneficial in severe forms of glomerular proliferative disorders.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Cell Proliferation*
  • Chemokine CXCL12 / biosynthesis
  • Epithelial Cells* / metabolism
  • Epithelial Cells* / pathology
  • Female
  • Glomerular Mesangium* / metabolism
  • Glomerular Mesangium* / pathology
  • Glomerulonephritis* / metabolism
  • Glomerulonephritis* / pathology
  • Humans
  • Inflammation Mediators / metabolism*
  • Male
  • Middle Aged
  • Peptidyl-Dipeptidase A / biosynthesis
  • Rats
  • Rats, Wistar
  • Receptor, Angiotensin, Type 1 / biosynthesis
  • Receptors, CXCR4 / biosynthesis
  • Stem Cells* / metabolism
  • Stem Cells* / pathology

Substances

  • CXCL12 protein, human
  • CXCL12 protein, rat
  • CXCR4 protein, human
  • CXCR4 protein, mouse
  • Chemokine CXCL12
  • Inflammation Mediators
  • Receptor, Angiotensin, Type 1
  • Receptors, CXCR4
  • ACE protein, human
  • Peptidyl-Dipeptidase A