Abstract
Chemically synthesised CH3Sp(A2'p)2A2'pp3'OCH3 has been used to assess the importance of the ppp(A2'p)nA (n greater than or equal to 2: 2-5A) system in the antiviral action of interferon against encephalomyocarditis virus (EMC). It inhibits activation of the 2-5A-dependent RNase by 2-5A in intact mouse L929 cells and cell-free systems. In interferon-treated, EMC-infected L929 cells it inhibits 2-5A-mediated rRNA cleavage and partially restores EMC RNA synthesis and virus yield. Activation of the 2-5A-dependent RNase must, therefore, play some part in interferon action against the growth of EMC virus in such cells.
MeSH terms
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Adenine Nucleotides / antagonists & inhibitors*
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Adenine Nucleotides / pharmacology
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Animals
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Cell-Free System
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Encephalomyocarditis virus / growth & development*
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Enzyme Activation / drug effects
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Interferons / antagonists & inhibitors*
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L Cells
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Mice
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Oligonucleotides / antagonists & inhibitors*
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Oligonucleotides / pharmacology*
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Oligoribonucleotides / antagonists & inhibitors*
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Oligoribonucleotides / pharmacology*
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Protein Biosynthesis
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RNA, Ribosomal / metabolism
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Ribonucleases / metabolism
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Thionucleotides / pharmacology*
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Virus Replication / drug effects*
Substances
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Adenine Nucleotides
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Oligonucleotides
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Oligoribonucleotides
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RNA, Ribosomal
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Thionucleotides
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2',5'-oligoadenylate
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Interferons
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Ribonucleases