Hydrogen-oxidizing hydrogenases 1 and 2 of Escherichia coli regulate the onset of hydrogen evolution and ATPase activity, respectively, during glucose fermentation at alkaline pH

FEMS Microbiol Lett. 2013 Nov;348(2):143-8. doi: 10.1111/1574-6968.12281. Epub 2013 Oct 10.

Abstract

Simultaneous measurement of redox potential (Eh ) and determination of H2 evolution kinetics using a pair of titanium silicate and platinum redox electrodes in fermenting cultures of Escherichia coli wild type and different mutants lacking hydrogenases 1 (Hyd-1) or 2 (Hyd-2) revealed that Hyd-1 controls the onset of H2 evolution at slightly alkaline pH (pH 7.5) and under oxidizing Eh . In addition, Hyd-2 influences the N,N'-dicyclohexylcarbodiimide-inhibited ATPase activity in fermenting cells and thus regulates the proton F0 F1 -ATPase at the alkaline pH but under reducing Eh .

Keywords: Escherichia coli; F0F1-ATPase; hydrogenase 1; hydrogenase 2; pH; redox potential.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism*
  • Fermentation / genetics
  • Glucose / metabolism*
  • Hydrogen / metabolism*
  • Hydrogen-Ion Concentration
  • Hydrogenase / genetics
  • Hydrogenase / metabolism*
  • Kinetics
  • Metabolic Networks and Pathways / genetics
  • Oxidation-Reduction

Substances

  • Escherichia coli Proteins
  • Hydrogen
  • Hydrogenase
  • Glucose