Abstract
An N-acetylhexosamine 1-kinase from Bifidobacterium infantis (NahK_15697), a guanosine 5'-diphosphate (GDP)-mannose pyrophosphorylase from Pyrococcus furiosus (PFManC), and an Escherichia coli inorganic pyrophosphatase (EcPpA) were used efficiently for a one-pot three-enzyme synthesis of GDP-mannose, GDP-glucose, their derivatives, and GDP-talose. This study represents the first facile and efficient enzymatic synthesis of GDP-sugars and derivatives starting from monosaccharides and derivatives.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bifidobacterium / enzymology*
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Escherichia coli / chemistry*
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Guanosine Diphosphate Mannose / chemical synthesis*
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Guanosine Diphosphate Mannose / chemistry
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Molecular Structure
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Monosaccharides / chemical synthesis*
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Monosaccharides / chemistry
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Phosphotransferases / chemistry*
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Phosphotransferases / metabolism
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Uridine Diphosphate Sugars / chemical synthesis*
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Uridine Diphosphate Sugars / chemistry*
Substances
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Monosaccharides
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Uridine Diphosphate Sugars
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Guanosine Diphosphate Mannose
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Phosphotransferases