Yeast three-hybrid screening for identifying anti-tuberculosis drug targets

Simone Moser; Kai Johnsson

doi:10.1002/cbic.201300472

Yeast three-hybrid screening for identifying anti-tuberculosis drug targets

Chembiochem. 2013 Nov 25;14(17):2239-42. doi: 10.1002/cbic.201300472. Epub 2013 Oct 16.

Authors

Simone Moser¹, Kai Johnsson

Affiliation

¹ Institute of Chemical Science and Engineering, Institute of Bioengineering, National Centre of Competence in Research NCCR Chemical Biology, École Polytechnique Fédérale de Lausanne, EPFL SB ISIC LIP, 1015 Lausanne (Switzerland); Current address: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139 (USA).

PMID: 24133019
DOI: 10.1002/cbic.201300472

Abstract

Mycobacterium goes yeast: Target deconvolution of anti-tuberculosis drugs can be a very challenging task. Here we report a yeast 3-hybrid system that allows promising small molecules to be screened for protein targets of a pathogen in nontoxic yeast cells. The system employs libraries of randomly fragmented bacterial DNA and offers a technically simple alternative approach for target identification.

Keywords: genomic library; snap-tag; target deconvolution; tuberculosis; yeast three-hybrid system.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antitubercular Agents / chemistry
Antitubercular Agents / pharmacology*
DNA, Bacterial / genetics
DNA, Bacterial / metabolism
Drug Evaluation, Preclinical*
Molecular Structure
Molecular Targeted Therapy / methods
Mycobacterium tuberculosis / drug effects*
Mycobacterium tuberculosis / genetics
Mycobacterium tuberculosis / metabolism*
Peptide Library
Small Molecule Libraries / chemistry
Small Molecule Libraries / pharmacology*
Structure-Activity Relationship
Two-Hybrid System Techniques*

Substances

Antitubercular Agents
DNA, Bacterial
Peptide Library
Small Molecule Libraries