Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazid

Haifeng Zeng; Jiadi Xu; Joseph Gillen; Michael T McMahon; Dmitri Artemov; Jean-Max Tyburn; Joost A B Lohman; Ryan E Mewis; Kevin D Atkinson; Gary G R Green; Simon B Duckett; Peter C M van Zijl

doi:10.1016/j.jmr.2013.09.012

Optimization of SABRE for polarization of the tuberculosis drugs pyrazinamide and isoniazid

J Magn Reson. 2013 Dec;237(100):73-78. doi: 10.1016/j.jmr.2013.09.012. Epub 2013 Sep 29.

Authors

Affiliations

¹ Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, USA. Electronic address: [email protected].
² Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, USA.
³ Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
⁴ Bruker BioSpin GmbH, Silberstreifen, Rheinstetten, Germany.
⁵ Bruker UK Limited, Banner Lane, Coventry, UK.
⁶ Centre for Hyperpolarisation in Magnetic Resonance, University of York, Heslington, York, UK.
⁷ Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA; F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, USA. Electronic address: [email protected].

Abstract

Hyperpolarization produces nuclear spin polarization that is several orders of magnitude larger than that achieved at thermal equilibrium thus providing extraordinary contrast and sensitivity. As a parahydrogen induced polarization (PHIP) technique that does not require chemical modification of the substrate to polarize, Signal Amplification by Reversible Exchange (SABRE) has attracted a lot of attention. Using a prototype parahydrogen polarizer, we polarize two drugs used in the treatment of tuberculosis, namely pyrazinamide and isoniazid. We examine this approach in four solvents, methanol-d4, methanol, ethanol and DMSO and optimize the polarization transfer magnetic field strength, the temperature as well as intensity and duration of hydrogen bubbling to achieve the best overall signal enhancement and hence hyperpolarization level.

Keywords: Hyperpolarization; Isoniazid; Mycobacterium Tuberculosis; Parahydrogen; Pyrazinamide; SABRE.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antitubercular Agents / chemistry*
Electromagnetic Fields
Hydrogen / chemistry
Isoniazid / chemistry*
Magnetic Resonance Spectroscopy / methods*
Pyrazinamide / chemistry*
Solvents

Substances

Antitubercular Agents
Solvents
Pyrazinamide
Hydrogen
Isoniazid

Abstract

Publication types

MeSH terms

Substances

Grants and funding