Molecular characterization of Candida spp. isolates from patients with bloodstream infections

Rev Soc Bras Med Trop. 2013 Nov-Dec;46(6):786-7. doi: 10.1590/0037-8682-1718-2013.

Abstract

Introduction: The aim of this study was to conduct an epidemiological study comparing the genetic similarity of yeasts isolated from blood cultures.

Methods: Random amplification of polymorphic DNA (RAPD) techniques were used for the Candida samples obtained from patients at the Hospital Universitário da Universidade Federal do Mato Grosso do Sul (HU/UFMS) in Campo Grande, State of Mato Grosso do Sul, Brazil, from 1998-2000.

Results: The most frequently isolated species was Candida albicans (45.8%). DNA amplification from genomic yeast isolates indicated a genetic similarity of over 90%.

Conclusions: The RAPD profiles obtained were able to differentiate between the isolated Candida species, thereby suggesting that the method might be useful in epidemiological studies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil / epidemiology
  • Candida / classification
  • Candida / genetics
  • Candida / isolation & purification
  • Candidiasis / epidemiology
  • Candidiasis / microbiology*
  • DNA, Fungal / genetics
  • Electrophoresis, Agar Gel
  • Fungemia / epidemiology
  • Fungemia / microbiology*
  • Humans
  • Mycological Typing Techniques
  • Random Amplified Polymorphic DNA Technique
  • Retrospective Studies

Substances

  • DNA, Fungal