IL-15 improves the cytotoxicity of cytokine-induced killer cells against leukemia cells by upregulating CD3+CD56+ cells and downregulating regulatory T cells as well as IL-35

Qianshan Tao; Tianping Chen; Lili Tao; Huiping Wang; Ying Pan; Shudao Xiong; Zhimin Zhai

doi:10.1097/CJI.0000000000000001

IL-15 improves the cytotoxicity of cytokine-induced killer cells against leukemia cells by upregulating CD3+CD56+ cells and downregulating regulatory T cells as well as IL-35

J Immunother. 2013 Nov-Dec;36(9):462-7. doi: 10.1097/CJI.0000000000000001.

Authors

Qianshan Tao¹, Tianping Chen, Lili Tao, Huiping Wang, Ying Pan, Shudao Xiong, Zhimin Zhai

Affiliation

¹ Department of Hematology, The Second Hospital Affiliated to Anhui Medical University, Hefei, Anhui, China.

PMID: 24145357
DOI: 10.1097/CJI.0000000000000001

Abstract

Cytokine-induced killer (CIK) cells are usually generated from peripheral blood mononuclear cells with the stimulation of IL-2 in vitro. Unlike the conventional IL-2-stimulated CIK cells (IL-2-CIK cells), we investigated the characteristics and potential mechanism of IL-15-stimulated CIK cells (IL-15-CIK cells) in this study. Compared with IL-2-CIK cells, the percentage of CD3CD56 cells was significantly increased in IL-15-CIK cells, but the expression of regulatory T (Treg) cells and IL-35 was significantly decreased in IL-15-CIK cells. Meanwhile, the in vitro cytotoxicity against human myeloid leukemia cells K562 of IL-15-CIK cells was significantly augmented compared with IL-2-CIK cells. These data suggest that IL-15 may improve the cytotoxicity of CIK cells against leukemia cells by upregulating CD3CD56 cells and downregulating Treg cells and IL-35.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
CD3 Complex / immunology
CD3 Complex / metabolism
CD56 Antigen / immunology
CD56 Antigen / metabolism
Cells, Cultured
Cytokine-Induced Killer Cells / immunology*
Cytotoxicity, Immunologic / drug effects
Cytotoxicity, Immunologic / immunology*
Down-Regulation / drug effects
Down-Regulation / immunology
Forkhead Transcription Factors / genetics
Forkhead Transcription Factors / metabolism
Gene Expression / drug effects
Gene Expression / immunology
Humans
Interleukin-15 / immunology*
Interleukin-15 / pharmacology
Interleukin-2 / immunology
Interleukin-2 / pharmacology
Interleukins / genetics
Interleukins / immunology*
Interleukins / metabolism
K562 Cells
Leukemia / immunology
Leukemia / pathology
Natural Killer T-Cells / drug effects
Natural Killer T-Cells / immunology*
Natural Killer T-Cells / metabolism
T-Lymphocytes, Regulatory / immunology*
T-Lymphocytes, Regulatory / metabolism
Up-Regulation / drug effects
Up-Regulation / immunology

Substances

CD3 Complex
CD56 Antigen
FOXP3 protein, human
Forkhead Transcription Factors
Interleukin-15
Interleukin-2
Interleukins
interleukin-35, human