Molecular characterization of Hysterothylacium fabri (Nematoda: Anisakidae) from Zeus faber (Pisces: Zeidae) caught off the Mediterranean coasts of Turkey based on nuclear ribosomal and mitochondrial DNA sequences

Parasitol Int. 2014 Feb;63(1):127-31. doi: 10.1016/j.parint.2013.10.006. Epub 2013 Oct 19.

Abstract

In the present study, Hysterothylacium fabri was found in the coasts of the Mediterranean Sea, Turkey and characterized by sequencing of nuclear (internal transcribed spacer, ITS) and mitochondrial (cytochrome c oxidase subunit 2, cox2) markers. Pairwise comparison between the entire ITS fragment including ITS-1, 5.8S, ITS-2 sequences of the H. fabri isolates from the Mediterranean Sea (Turkey, KC852206) and other H. fabri isolates from the South China Sea (JQ520158), the South Korea waters (JX974558) showed differences ranged from 0.1 and 1.1%. With the present study, H. fabri from the Mediterranean Sea was characterized for the first time by sequencing of the cox2 gene.

Keywords: Hysterothylacium fabri; Mediterranean Sea; Mitochondrial DNA cox2 gene; Ribosomal DNA ITS region; Sequencing.

MeSH terms

  • Animals
  • DNA, Mitochondrial / genetics*
  • DNA, Ribosomal / genetics*
  • Fish Diseases / epidemiology
  • Fish Diseases / parasitology*
  • Fishes
  • Mediterranean Sea / epidemiology
  • Nematoda / genetics*
  • Nematode Infections / epidemiology
  • Nematode Infections / veterinary*
  • Phylogeny
  • Turkey

Substances

  • DNA, Mitochondrial
  • DNA, Ribosomal