Phorbol ester enhances deoxynucleoside incorporation while inhibiting proliferation of K-562 cells

Cytometry. 1985 Nov;6(6):591-6. doi: 10.1002/cyto.990060614.

Abstract

Recently a new technique has been developed to study bromodeoxyuridine incorporation into DNA as a function of the cell cycle phase distribution. We have made the observation that treatment of the hematopoietic cell line K-562 results in a complete and immediate cessation of cell growth, although tritiated thymidine incorporation is markedly elevated for as long as 5 days. We have made use of that newly described technique to study this phenomenon.

MeSH terms

  • Bromodeoxyuridine
  • Cell Cycle / drug effects*
  • Cell Line
  • DNA / biosynthesis*
  • Deoxyribonucleosides / metabolism*
  • Flow Cytometry
  • Humans
  • Interphase
  • Phorbols / pharmacology*
  • Protein Biosynthesis
  • RNA / biosynthesis
  • Tetradecanoylphorbol Acetate / pharmacology*

Substances

  • Deoxyribonucleosides
  • Phorbols
  • RNA
  • DNA
  • Bromodeoxyuridine
  • Tetradecanoylphorbol Acetate