An assay to quantify chemotactic properties of degradation products from extracellular matrix

Methods Mol Biol. 2014:1202:103-10. doi: 10.1007/7651_2013_37.

Abstract

The endogenous chemotaxis of cells toward sites of tissue injury and/or biomaterial implantation is an important component of the host response. Implanted biomaterials capable of recruiting host stem/progenitor cells to a site of interest may obviate challenges associated with cell transplantation. An assay for the identification and quantification of chemotaxis induced by surgically placed biologic scaffolds composed of extracellular matrix is described herein.

MeSH terms

  • Animals
  • Biological Assay / instrumentation
  • Biological Assay / methods*
  • Chemotactic Factors / pharmacology
  • Chemotaxis* / drug effects
  • Extracellular Matrix / drug effects
  • Extracellular Matrix / metabolism*
  • Filtration
  • Rats

Substances

  • Chemotactic Factors