Genome-wide effects of DNA methyltransferase inhibitor on gene expression in double-stranded RNA transfected porcine PK15 cells

Xiaoshuo Wang; Hong Ao; Liwei Zhai; Lijing Bai; Weiyong He; Ying Yu; Chuduan Wang

doi:10.1016/j.ygeno.2013.10.005

Genome-wide effects of DNA methyltransferase inhibitor on gene expression in double-stranded RNA transfected porcine PK15 cells

Genomics. 2014 May-Jun;103(5-6):371-9. doi: 10.1016/j.ygeno.2013.10.005. Epub 2013 Oct 31.

Authors

Xiaoshuo Wang¹, Hong Ao², Liwei Zhai¹, Lijing Bai², Weiyong He³, Ying Yu⁴, Chuduan Wang⁵

Affiliations

¹ National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China.
² State Key Laboratory for Animal Nutrition, Key Laboratory for Domestic Animal Genetic Resources and Breeding of the Ministry of Agriculture of China, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
³ Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
⁴ National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China. Electronic address: [email protected].
⁵ National Engineering Laboratory for Animal Breeding, Key Laboratory of Agricultural Animal Genetics and Breeding, Department of Animal Breeding and Genetics, College of Animal Sciences and Technology, China Agricultural University, Beijing 100193, China. Electronic address: [email protected].

PMID: 24184360
DOI: 10.1016/j.ygeno.2013.10.005

Abstract

Double-stranded RNA (dsRNA) is produced in host cells during viral replication. The effects of DNA demethylation on gene expression in dsRNA transfected swine cells are unclear. The study aims to profile the transcriptome changes which are induced by DNA methyltransferase inhibitor (Aza-CdR) in porcine PK15 cells transfected with viral-like dsRNA (Poly(I:C)). A total of 44, 76 and 952 differentially expressed genes (DEGs) were detected in the cells treated by Poly(I:C) plus Aza-CdR (P+A), Poly(I:C) (P) or Aza-CdR (A) alone compared to the controls (C). Immune response-related pathways are observed in the comparison of A vs. C and P vs. C, and the genes in the pathways were recovered in the comparison of (P+A) vs. C. GO analysis indicated that Aza-CdR has negative regulatory effects on viral reproduction. The results suggest that the stimulant of Poly(I:C) could be regressed by Aza-CdR. These observations provide new insights into the epigenetic regulatory effects on viral replication.

Keywords: DNA demethylation; Double-stranded RNA; Transcriptome; Transfection; Virus replication.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Azacitidine / analogs & derivatives*
Azacitidine / pharmacology
Cell Line
DNA Modification Methylases / antagonists & inhibitors*
Decitabine
Genome
Interferon-alpha / genetics
Interferon-alpha / metabolism
Oligonucleotide Array Sequence Analysis
Poly I-C / pharmacology*
RNA, Double-Stranded / genetics*
Signal Transduction
Sus scrofa
Toll-Like Receptor 3 / genetics
Toll-Like Receptor 3 / metabolism
Transcriptome / drug effects*
Transfection

Substances

Interferon-alpha
RNA, Double-Stranded
Toll-Like Receptor 3
Decitabine
DNA Modification Methylases
Azacitidine
Poly I-C