Comparison of frozen and unfrozen blood spots for gene expression studies

J Pediatr. 2014 Jan;164(1):189-191.e1. doi: 10.1016/j.jpeds.2013.09.025. Epub 2013 Oct 25.

Abstract

We studied gene expression in 9 sets of paired newborn blood spots stored for 8-10 years in either the frozen state or the unfrozen state. Fewer genes were expressed in unfrozen spots, but the average correlation coefficient for overall gene expression comparing the frozen and unfrozen state was 0.771 (95% CI, 0.700-0.828).

Keywords: ELGAN; Extremely Low Gestational Age Newborns.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Blood Specimen Collection
  • Cryopreservation*
  • Freezing*
  • Gene Expression Profiling / methods*
  • Humans
  • Infant, Newborn
  • Neonatal Screening*
  • Oligonucleotide Array Sequence Analysis / methods*
  • RNA, Messenger / blood*
  • Time Factors

Substances

  • RNA, Messenger