The distinctive germinal center phase of IgE+ B lymphocytes limits their contribution to the classical memory response

J Exp Med. 2013 Nov 18;210(12):2755-71. doi: 10.1084/jem.20131539. Epub 2013 Nov 11.

Abstract

The mechanisms involved in the maintenance of memory IgE responses are poorly understood, and the role played by germinal center (GC) IgE(+) cells in memory responses is particularly unclear. IgE(+) B cell differentiation is characterized by a transient GC phase, a bias toward the plasma cell (PC) fate, and dependence on sequential switching for the production of high-affinity IgE. We show here that IgE(+) GC B cells are unfit to undergo the conventional GC differentiation program due to impaired B cell receptor function and increased apoptosis. IgE(+) GC cells fail to populate the GC light zone and are unable to contribute to the memory and long-lived PC compartments. Furthermore, we demonstrate that direct and sequential switching are linked to distinct B cell differentiation fates: direct switching generates IgE(+) GC cells, whereas sequential switching gives rise to IgE(+) PCs. We propose a comprehensive model for the generation and memory of IgE responses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Cell Differentiation
  • Germinal Center / cytology
  • Germinal Center / immunology
  • Green Fluorescent Proteins / genetics
  • Immunoglobulin Class Switching
  • Immunoglobulin E / metabolism*
  • Immunoglobulin G / metabolism
  • Immunologic Memory*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Transgenic
  • Models, Immunological*
  • Nippostrongylus
  • Plasma Cells / cytology
  • Plasma Cells / immunology
  • Receptors, Antigen, B-Cell / metabolism
  • Signal Transduction
  • Strongylida Infections / immunology

Substances

  • Immunoglobulin G
  • Receptors, Antigen, B-Cell
  • Green Fluorescent Proteins
  • Immunoglobulin E