Iloprost reduces colitis induced oxidative stress: An experimental study in rats

Turk J Gastroenterol. 2013;24(3):224-9. doi: 10.4318/tjg.2013.0491.

Abstract

Background/aims: Reactive oxygen species have a known potent role in the pathogenesis of ulcerative colitis. Iloprost, a pharmaceutical, is a chemically stable derivative of a naturally- occurring human prostacyclin. Several studies have demonstrated protective effects of iloprost via its antioxidant and its anti-inflammatory activity. The aim of this study is to evaluate the effects of iloprost on oxidant/antioxidant status, as well as the large bowel histopathology in experimental colitis.

Materials and method: Forty adult male Wistar-albino rats were randomly divided in to four equal weight-matched groups: sham group (n=10), iloprost administered sham group (n=10), colitis group (n=10), iloprost administered colitis group (n=10). Acetic acid (1 ml of 4% solution) was used to induce colonic inflammation in the rats.

Results: Colonic tissue and plasma malondialdehyde levels were significantly lower in the iloprost administered colitis group than the colitis group (p<0.01). Tissue glutathione levels of the iloprost administered colitis group were significantly higher than the colitis group (p<0.001).

Conclusion: We have demonstrated in this study iloprost to be an antioxidant, as well as iloprost demonstrating protective activity against colitis induced oxidative stress.

MeSH terms

  • Acetic Acid
  • Animals
  • Antioxidants / pharmacology*
  • Colitis / chemically induced
  • Colitis / drug therapy*
  • Colitis / metabolism
  • Colitis / pathology
  • Colon / metabolism
  • Glutathione / metabolism
  • Iloprost / pharmacology*
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress / drug effects*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • Malondialdehyde
  • Superoxide Dismutase
  • Glutathione
  • Iloprost
  • Acetic Acid