Significance of CD66c expression in childhood acute lymphoblastic leukemia

Nobutaka Kiyokawa; Kazutoshi Iijima; Osamu Tomita; Masashi Miharu; Daisuke Hasegawa; Kenichiro Kobayashi; Hajime Okita; Michiko Kajiwara; Hiroyuki Shimada; Takeshi Inukai; Atsushi Makimoto; Takashi Fukushima; Toru Nanmoku; Katsuyoshi Koh; Atsushi Manabe; Akira Kikuchi; Kanji Sugita; Junichiro Fujimoto; Yasuhide Hayashi; Akira Ohara

doi:10.1016/j.leukres.2013.10.008

Significance of CD66c expression in childhood acute lymphoblastic leukemia

Leuk Res. 2014 Jan;38(1):42-8. doi: 10.1016/j.leukres.2013.10.008. Epub 2013 Oct 22.

Authors

Nobutaka Kiyokawa¹, Kazutoshi Iijima², Osamu Tomita², Masashi Miharu³, Daisuke Hasegawa⁴, Kenichiro Kobayashi², Hajime Okita², Michiko Kajiwara⁵, Hiroyuki Shimada⁶, Takeshi Inukai⁷, Atsushi Makimoto⁸, Takashi Fukushima⁹, Toru Nanmoku¹⁰, Katsuyoshi Koh¹¹, Atsushi Manabe¹², Akira Kikuchi¹³, Kanji Sugita⁷, Junichiro Fujimoto¹⁴, Yasuhide Hayashi¹⁵, Akira Ohara¹⁶

Affiliations

¹ Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan. Electronic address: [email protected].
² Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan.
³ Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan; Department of Pediatrics, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
⁴ Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan; Department of Pediatrics, St. Luke's International Hospital, Chuo-ku, Tokyo, Japan.
⁵ Department of Transfusion Medicine, Medical Hospital, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan.
⁶ Department of Pediatrics, Keio University School of Medicine, Shinjuku-ku, Tokyo, Japan.
⁷ Department of Pediatrics, School of Medicine, University of Yamanashi, Chuo, Yamanashi, Japan.
⁸ Division of Pediatric Oncology, National Cancer Center Hospital, Chuo-ku, Tokyo, Japan.
⁹ Department of Child Health, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
¹⁰ Department of Clinical Laboratory, University of Tsukuba Hospital, Tsukuba, Ibaraki, Japan.
¹¹ Department of Hematology/Oncology, Saitama Children's Medical Center, Saitama, Saitama, Japan.
¹² Department of Pediatrics, St. Luke's International Hospital, Chuo-ku, Tokyo, Japan.
¹³ Department of Pediatrics, Teikyo University School of Medicine, Itabashi-ku, Tokyo, Japan.
¹⁴ Clinical Research Center, National Center for Child Health and Development, Setagaya-ku, Tokyo, Japan.
¹⁵ Department of Hematology-Oncology, Gunma Children's Medical Center, Shibukawa, Gunma, Japan.
¹⁶ Department of Pediatrics, Toho University Omori Medical Center, Ota-ku, Tokyo, Japan.

PMID: 24231528
DOI: 10.1016/j.leukres.2013.10.008

Abstract

Upon analyzing 696 childhood B-cell precursor acute lymphoblastic leukemia (BCP-ALL) cases, we identified the characteristics of CD66c expression. In addition to the confirmation of strong correlation with BCR-ABL positivity and hyperdiploid, we further observed that CD66c is frequently expressed in CRLF2-positive (11/15, p<0.01 against chimeric gene-negative) as well as hypodiploid cases (3/4), whereas it is never expressed in ETV6-RUNX1, MLL-AF4, MLL-AF9, MLL-ENL, and E2A-PBX1-positive cases. Although the expression of CD66c itself is not directly linked to the prognosis, the accompanying genetic abnormalities are important prognostic factors for BCP-ALL, indicating the importance of CD66c expression in the initial diagnosis of BCP-ALL.

Keywords: Acute lymphoblastic leukemia; CD66c; CRLF2, Flow cytometry; Genetic abnormality.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Antigens, CD / metabolism*
Cell Adhesion Molecules / metabolism*
Child
Child, Preschool
Core Binding Factor Alpha 2 Subunit / genetics
Female
Flow Cytometry
Fusion Proteins, bcr-abl / genetics
GPI-Linked Proteins / metabolism
Gene Expression Regulation, Neoplastic
Homeodomain Proteins / genetics
Humans
Infant
Infant, Newborn
Male
Myeloid-Lymphoid Leukemia Protein / genetics
Oncogene Proteins, Fusion / genetics*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics
Precursor Cell Lymphoblastic Leukemia-Lymphoma / metabolism*
Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
Prognosis
Receptors, Cytokine / metabolism*
Reverse Transcriptase Polymerase Chain Reaction

Substances

Antigens, CD
CEACAM6 protein, human
CRLF2 protein, human
Cell Adhesion Molecules
Core Binding Factor Alpha 2 Subunit
GPI-Linked Proteins
Homeodomain Proteins
MLL-AF4 fusion protein, human
MLL-AF9 fusion protein, human
MLL-ENL oncoprotein, human
Oncogene Proteins, Fusion
Receptors, Cytokine
TEL-AML1 fusion protein
E2A-Pbx1 fusion protein
Myeloid-Lymphoid Leukemia Protein
Fusion Proteins, bcr-abl