Periodic acid-ethanol gelatin methenamine silver stain for demonstrating M. leprae and myelin in peripheral nerve fibers of leprosy patients

Int J Lepr Other Mycobact Dis. 1986 Mar;54(1):84-7.

Abstract

We have introduced the periodic acid-ethanol gelatin methenamine silver stain for the simultaneous demonstration of Mycobacterium leprae and myelin in the peripheral nerves of leprosy patients.

MeSH terms

  • Ethanol
  • Gelatin
  • Humans
  • Leprosy / microbiology*
  • Leprosy / pathology
  • Methenamine
  • Mycobacterium leprae / isolation & purification*
  • Myelin Sheath / pathology*
  • Nerve Fibers / microbiology*
  • Nerve Fibers / pathology
  • Periodic Acid
  • Skin / innervation*
  • Spinal Nerves / microbiology*
  • Staining and Labeling
  • Sural Nerve / microbiology*
  • Sural Nerve / pathology

Substances

  • Periodic Acid
  • Ethanol
  • Gelatin
  • Methenamine