Overexpression of Bcl-3 inhibits the development of marginal zone B cells

Eur J Immunol. 2014 Feb;44(2):545-52. doi: 10.1002/eji.201343655. Epub 2013 Nov 18.

Abstract

The transcription factor Bcl-3 functions as a proto-oncogene via regulation of cell proliferation and apoptosis. Bcl-3 is an atypical member of the IκB family and plays a central role in the immune response through interactions with the NF-κB subunits p50 and p52. To investigate the impact of Bcl-3 on B-cell maturation and regulation, we generated mice that overexpress Bcl-3 specifically in B cells. Interestingly, these mice lack marginal zone B cells and exhibit a significant reduction in the number of B-1 B cells. Further, B cells from these mice are impaired in their proliferative capacity. Our data demonstrate that the overexpression of the transcription factor Bcl-3 inhibits germinal center formation, marginal zone B-cell development, and affects the B-1 B-cell compartment.

Keywords: B-1 B cells; Bcl-3; Marginal zone B cell; NF-κB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • B-Cell Lymphoma 3 Protein
  • B-Lymphocytes / metabolism*
  • Cell Proliferation
  • Gene Expression / genetics*
  • Germinal Center / metabolism
  • Mice
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins / metabolism*
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism*

Substances

  • B-Cell Lymphoma 3 Protein
  • Bcl3 protein, mouse
  • Proto-Oncogene Proteins
  • Transcription Factors