Abstract
Piroplasmosis is a serious debilitating and sometimes fatal disease. Phylogenetic relationships within piroplasmida are complex and remain unclear. We compared the intron-exon structure and DNA sequences of the RPS8 gene from Babesia and Theileria spp. isolates in China. Similar to 18S rDNA, the 40S ribosomal protein S8 gene, RPS8, including both coding and non-coding regions is a useful and novel genetic marker for defining species boundaries and for inferring phylogenies because it tends to have little intra-specific variation but considerable inter-specific difference. However, more samples are needed to verify the usefulness of the RPS8 (coding and non-coding regions) gene as a marker for the phylogenetic position and detection of most Babesia and Theileria species, particularly for some closely related species.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Babesia / classification*
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Babesia / genetics
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Base Sequence
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Cattle
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China
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DNA, Protozoan / classification*
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DNA, Protozoan / genetics
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Exons
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Genetic Markers
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Humans
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Introns
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Molecular Sequence Data
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Open Reading Frames
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Phylogeny*
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Protozoan Proteins / classification*
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Protozoan Proteins / genetics
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Ribosomal Proteins / classification*
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Ribosomal Proteins / genetics
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Ribosome Subunits, Small, Eukaryotic / chemistry
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Theileria / classification*
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Theileria / genetics
Substances
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DNA, Protozoan
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Genetic Markers
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Protozoan Proteins
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Ribosomal Proteins
Grants and funding
This work was supported, in part, by the National Natural Sciences Foundation of China (numbers 31201899 and 30972182), the Natural Science Foundation of Gansu Province (number 096RJZA128), the Science Fund for Creative Research Groups Of Gansu Province (number 1210RJIA006), Key Project of Gansu Province (092NKDA031). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.