Target-induced quenching for highly sensitive detection of nucleic acids based on label-free luminescent supersandwich DNA/silver nanoclusters

Guangfeng Wang; Yanhong Zhu; Ling Chen; Lun Wang; Xiaojun Zhang

doi:10.1039/c3an01702h

Target-induced quenching for highly sensitive detection of nucleic acids based on label-free luminescent supersandwich DNA/silver nanoclusters

Analyst. 2014 Jan 7;139(1):165-9. doi: 10.1039/c3an01702h. Epub 2013 Nov 15.

Authors

Guangfeng Wang¹, Yanhong Zhu, Ling Chen, Lun Wang, Xiaojun Zhang

Affiliation

¹ Key Laboratory of Chem-Biosensing, College of Chemistry and Materials Science, Center for Nano Science and Technology, Anhui Normal University, Wuhu 241000, Anhui Province, P.R. China. [email protected].

PMID: 24244937
DOI: 10.1039/c3an01702h

Abstract

Luminescent silver nanoclusters (AgNCs) were anchored by designed oligonucleotides, acting as fluorescent labels. They hybridized with specific nucleic acid targets to form a supersandwich structure resulting in the fluorescence intensity of the DNA/AgNCs decreasing linearly with respect to the concentration of the target DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
Limit of Detection
Luminescent Measurements / methods*
Luminescent Measurements / standards
Metal Nanoparticles / chemistry*
Nucleic Acids / analysis*
Silver / chemistry*

Substances

Nucleic Acids
Silver
DNA